Thioflavin T (Th T) is a dye which shows enhanced fluorescence at around 480 nm when bound to amyloid fibrils. 34 Thus to investigate and compare the aggregates formed by incubated -lg and ox--lg at 37 C, 65 C and 80 C for 4 h, the following Th T assay was employed.Methionine (Met) is believed to be one of the most readily oxidisable amino acid residues of protein.Bovine beta-lactoglobulin (-lg), a model globular whey protein, has been presented as a subsequent paradigm for studies on protein aggregation and amyloid formation.
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